非水反相液相色谱法测定大鼠虎杖给药后血浆中大黄素、大黄素甲醚的含量  被引量：7

作　　者：郝晓花[1] 蒋晔[1] 姜少灏[1] 刘红菊[1] 冀国荣[1] 

机构地区：[1]河北医科大学药学院,河北石家庄050017

出　　处：《中成药》2005年第4期453-455,共3页Chinese Traditional Patent Medicine

摘　　要：目的:采用非水反相液相色谱法测定大鼠血浆中大黄素、大黄素甲醚的含量.方法:血浆经水解、提取后用非水反相高效液相色谱法分析其含量,采用Kromasil C18色谱柱(250mm×4.6mm,5.0μm),甲醇-冰醋酸(99.9:0.1)为流动相;流速为1.0mL·min-1;检测波长254nm.结果:大黄素在0.0425～2.8μg·mL-1、大黄素甲醚在0.0491～3.14μg·mL-1范围内线性关系良好;大黄素、大黄素甲醚的回收率分别为95.7%～100.1%,96.2%～99.8%,RSD分别为1.3%,1.6%;提取回收率分别为91.8%～95.4%、92.7%～94.6%.结论:本方法准确、简便,重现性好,适宜于测定含大黄素、大黄素甲醚的生物样品.AIM: To determine the content of emodin and physcion in rat plasma by nonaqueous RP-HPLC. METHODS: After hydrolysis and extraction, the content of emodin in the plasma was determined by nonaqueous RP-HPLC. The separation was performed on Kromasil C 18 column (250 mm×4.6 mm, 5.0 μm) with the mobile phase comprised of methanol-acetic acid (99.9∶0.1). The flow rate was 1.0 mL·min -1 and the detection wavelength was at 254 nm. RESULTS: The linear ranges for emodin and physcion were in the range of 0.0425-2.8 μg·mL -1 and 0.0491-3.14 μg·mL -1 , respectively. The average recoveries of emodin and physcion were 95.7%-100.1% and 96.2%-99.8%, with corresponding RSD of 1.3% and 1.6% respectively. CONCLUSION: This method is simple, rapid, accurate and reproducible with RP-HPLC to detect rhein in plasma.

关 键 词：大黄素 大黄素甲醚 非水反相液相色谱法 

分 类 号：R945[医药卫生—微生物与生化药学]